Inoculating a Grain Bag: The First Step to Growing Mushrooms

How to Inoculate a Grain Bag (Using Live Culture)

Inoculating a grain bag is the first step in gourmet mushroom cultivation. Using liquid culture, this process introduces live mycelium into a nutrient-rich environment to begin colonization.

In this guide, we’ll show you how to do it right—from prepping your space to injecting your grain bag—so you get clean, strong growth every time.

✅ Learn how to avoid contamination✅ Understand proper sterile technique✅ Set yourself up for a successful colonization

Once your bag is fully colonized, check out our Grain-to-Grain Transfer Guide to expand your culture and scale your grow.

💬 Have questions? Reach out:📩 contact@sporevaultco.com

Inoculating 5LB grain bag with spore syringe